Salt, Cold, and Drought Stress on Einkorn and Bread Wheat during Germination

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Nusret Zencirci Hakan Ulukan Bülent Ordu Didem Aslan Hakan Tahiri Mutlu Mehmet Örgeç


Climate changes prompt salt, cold, and drought stresses especially during early crop growth stages. The damages during germination in wheat may even destroy whole crop. Here, 12 bread and 10 einkorn wheats entries were distressed under salt, cold and drought.  Germination rate and germination power, coleoptile, shoot length, root length, shoot to root length ratio, root fresh and dry weight and root fresh to dry weight ratio were quantified under six salt, cold, drought stresses and one control. After ANOVA and LSD discriminated the entries, stress tolerance indices differentiated six tolerant and six susceptible entries. MANOVA, Pillai’s Trace and Wilks’ Lambda tests finalized the stress testing. Shoot and root length, root fresh and dry weight highly differed under salt, cold and drought. Bayraktar-2000 well tolerated salt, drought, salt-drought and salt-cold-drought; Gerek-79 salt, salt-drought, salt-cold-drought; Momtchil salt, cold and salt-cold-drought; İkizce-96 salt, drought and salt-drought. Einkorn Population 14 was susceptible to all stresses except cold and salt-cold; Population 15 to salt, salt-drought and salt-cold-drought; Population 11 to salt, drought, salt-drought and salt-cold-drought. These stresses sharply decreased shoot and root length, root fresh and root dry weight. The higher % decreases under salt, cold and drought were in shoot (59.72, 63.25 and 23.17) and root length (32.91, 51.77 and 34.69), root fresh (44.32, 49.11 and 38.88) and root dry weight (21.63, 42.14 and 41.97). Moreover, Pillai’s trace and Wilks’ Lambda tests differentiated both characters and entries (P < 0.01). In conclusion, Momtchil, Gerek-79, Bayraktar-2000, Populations 5, 6, and 1 are well endorsed against triple seedling stresses.

Article Details

Sending to International Journal of Secondary Metabolite
Author Biographies

Hakan Ulukan, Ankara University

Ankara Univ., Agricultural Faculty, Field Crops Dept., Dışkapı, Ankara, Turkey

Bülent Ordu, Bolu Abant İzzet Baysal University

Bolu Abant İzzet Baysal Univ., Economics and Administrative Sciences Faculty, Business Admin. Dept., 14200, Gölköy, Bolu, Turkey

Didem Aslan, Bolu Abant İzzet Baysal University

Bolu Abant İzzet Baysal Univ., Sciences and Art Faculty, Biology Dept., 14200, Gölköy, Bolu, Turkey

Hakan Tahiri Mutlu, Bolu Abant İzzet Baysal University

Bolu Abant İzzet Baysal Univ., Economics and Administrative Sciences Faculty, Business Admin. Dept., 14200, Gölköy, Bolu, Turkey

Mehmet Örgeç, Bolu Abant İzzet Baysal University

Bolu Abant İzzet Baysal Univ., Sciences and Art Faculty, Biology Dept., 14200, Gölköy, Bolu, Turkey


[1] Dhanda, S.S., Sethi, G.S., Behl, R.K. (2004). Indices of Drought Tolerance in Wheat Genotypes at Early Stages of Plant Growth. J. Agron Crop Sci., 190, 6-12.

[2] Feuillet, C., Langridge, P., Waugh, R. (2007). Cereal Breeding Takes A Walk on the Wild Side. Trends Genet., 24(1), 1–32.

[3] Shahzad, A., Iqbal, M., Asif, M., Hirani, A.H., Goyal A. (2013). Growing Wheat on Saline Lands, Can A Dream Come True? Australian J. Crop Sci., 7, 515-524.

[4] Eren, H, Pekmezci, M.Y, Okay, S., Turktas, M., Inal, B., Ilhan, E., Atak, M., Erayman, M., Unver, T., Unver C.T. (2015). Hexaploid Wheat (Triticum Aestivum) Root Mirnome Analysis in Response to Salt Stress. Ann Appl Biol., 167, 2-30.

[5] Charmet, G. (2011). Wheat Domestication: Lessons for the Future. C. R. Biologies, 334, 212-220.

[6] Hidalgo, A., Brandolini, A. (2013). Nutritional Properties of Einkorn Wheat (Triticum monococcum L.). J. Sci. Food Agric., 94, 601–61.

[7] Sharma, H.C., Waines, J.G., Foster, W. (1981). Variability in Primitive and Wild Wheats for Useful Genetic Characters. Crop Sci., 21, 555–559.

[8] Kranner, I, Minibayeva, F.V., Beckett, R.P., Seal C.E., (2010). What Is Stress? Concepts, Definitions and Applications in Seed Science. Tansley Rev. New Phytol, 188, 655 - 673.

[9] Mehrotra, R., Bhalothia, P., Bansal, P., Basantani, M.K., Bharti, V., Mehrotra, S. (2014). Abscisic Acid and Abiotic Stress Tolerance-Different Tiers of Regulation. J. Plant Phys., 171, 486 - 496.

[10] Pierik, R., Testerink, C. (2014). The Art of Being Flexible, How to Escape from The Shade, Salt, And Drought. J Plant Phys., 166, 5-22.

[11] Izadi, M.H., Rabbani, J., Emam, Y., Pessarakli, M., Tahmasebi, A. (2014). Effects of Salinity Stress on The Physiological Performance of Various Wheat and Barley Cultivars. J. Plant Nutr, 37, 520 - 531.

[12] Richter, J.E., Kopka, J., Zerb, C. (2015). Metabolic Contribution to Sal t Stress in Two Maize Hybrids with Contrasting Resistance. Plant Sci., 233, 107-115.

[13] Hasanuzzaman, M., Nahar, K., Mahabub, A.M.D., Bhowmik, C.P., Amzad, H.M.D., Rahman, M.M., Prasad, V., Narasimha, M., Ozturk, M., Fujita M. (2014). Potential Use of Halophytes to Remediate Saline Soils. BioMed Res. Int., 1-12.

[14] Vardar, Y., Çifci E.A. (2014). Salinity Effects on Germination Stage of Bread and Durum Wheat Cultivars. J Yuzuncu Yıl Univ., 24, 127-139.

[15] Braun, H.J., Ekiz, H., Eser, V., Keser, M., Ketata, H., Marcucci, G., Morgounov, A.I., Zencirci N. (1998). Breeding Priorities of Winter Wheat Programs. In: H.-J. Braun, F. Altay, W.E. Kronstad, S.P.S. Beniwal & A. McNab, editors. Wheat, Prospects for Global Improvement. Proc. 5th Int. Wheat Conf., Ankara, Developments in Plant Breeding, Kluwer Academic Publishers, Dordrecht, Netherlands. pp 553 – 560.

[16] Khodabandeh, N. (2003). Cereals. Seventh Edition, Tehran University Press, pp. 78- 111.

[17] Mostek, A., Börner, A., Badowiec, A., Weidner, S. (2015). Alterations in Root Proteome of Salt-Sensitive and Tolerant Barley Lines under the Salt Stress Conditions. J. Plant Phys., 174, 166-176.

[18] Karakaş, F.P. (2016). Effects of Drought and Salinity Stress on Early Seedling Growth and Antioxidant Activity in Hulled Einkorn (Triticum monococcum ssp. monococcum) and Bread (Triticum aestivum L.) Wheats. J. CRI for Field Crops, 25, 107-116.

[19] Aslan, D., Zencirci, N., Etöz, M., Ordu, B., Bataw S. (2016). Bread Wheat Responds Salt Stress Better Than Einkorn Wheat Does During Germination. Turkish J. Agric. For., 40(5), 783-794.

[20] Aslan, D., Ordu, B., Zencirci, N. (2016). Einkorn Wheat (Triticum monococcum ssp. monococcum) Tolerates Cold Stress Better than Bread Wheat (Triticum aestivum L.) During Germination. J. Field Crops Cent. Res. Inst., 25(2), 182-192.

[21] Ashraf, M., Harris P.J.C. (2013). Photosynthesis under Stressful Environments: An Overview. Photosynth, 51(2), 163-190.

[22] Baloch, M.J., Dunwell, J., Khakwani, A.A., Dennett, M., Jatoi, W.A., Channa S.A. (2012). Assessment of Wheat Cultivars for Drought Tolerance via Osmotic Stress Imposed at Early Seedling Growth Stages. J. Agric. Res., 50(3), 299 - 310.

[23] Thornley, J.M. (1998). Modelling Shoot, Root Relations, The Way Forward. Ann Bot., 81, 165-171.

[24] Zencirci, N., Eser, V., Baran, I. (1990). Comparison of Some Stability Statistics. CRIFC Publicaitions, Publication no, 2:17 (in Turkish).

[25] Gomez, K., Gomez, A.A. (1984). Statistical Procedures for Agricultural Research, 2nd edition. John Wiley and Sons: New York, USA. pp. 680

[26] Kalaycı, Ş. (2010). SPSS Uygulamalı Çok Değişkenli İstatistik Teknikleri: In: ASİL Yayın Dağıtım Ltd. Şti., Ankara, Turkey P. 116 (SPSS applied multi-variate statistic techniques. Pg 116. ASİL Publication Casting Ltd. Co. Ankara, Turkey) [in Turkish].

[27] Askari, H., Kazemitabar, S.K., Zarrini, H.N., Saberi M.H. (2016). Salt Tolerance Assessment of Barley (Hordeum vulgare L.). Open Agric., 1, 37-44.

[28] Oyiga, B.C., Sharma, R.C., Shen, J., Baum, M., Ogbonnaya, F.C., Leon, J., Ballvora, A. (2016). Identification and Characterization of Salt Tolerance of Wheat Germplasm Using A Multivariable Screening Approach. J Agron Crop Sci., 202, 472 - 485.

[29] Khan, M.A., Ungar, I.A., Showalter A.M. (2000). Effects of Salinity on Growth, Water Relations and Ion Accumulation of The Subtropical Perennial Halophyte, Atriplex griffithii var. stocksii. Ann. Bot., 31, 2763-2774.

[30] Rahman, M., Soomro, U.A., Zahoor-ul-Haq, M., Gul, S. (2008). Effects of NaCl Salinity on Wheat (Triticum aestivum L.) Cultivars. World J. Agric. Sci., 4, 398-403.

[31] Aslan, D., Aktaş, H., Ordu, B., Zencirci, N., (2017). Evaluation of Bread and Einkorn Wheat Under in vitro Drought Stress. The J. Animal Plant Sci. 27(6), 1974-1983.

[32] Mahmoodabad, R.Z., Somarin, S.J., Khayatnezhad, M., Gholamin, R. (2001). Effect of Cold Stress on Germination and Growth of Wheat Cultivars. Adv. Environ. Biol. 5, 94–97.

[33] Foolad, M.R., Lin, G.Y. (1997). Genetic Potential for Salt Tolerance During Germination in Lycopersicon Species. Hort. Sci., 32, 296-300.

[34] Foolad, M.R., Lin, G.Y. (1998). Genetic Analysis of Low Temperature Tolerance during Germination in Tomato, Lycopersicon Esculentum, Mill. Plant Breed., 117, 171-176.

[35] Mahmoodzadeh, H., Masoudi, F.K., Besharat, H., (2013). Impact of Salt Stress on Seed Germination Indices of Five Wheat Cultivars. Ann. Biol. Res., 4, 93-96.

[36] Ali, M.B., El-Sadek, A.N. (2016). Evaluation of Drought Tolerance Indices for Wheat (Triticum aestivum L.) Under the Irrigated and Rainfed Conditions. Commun Biometry Crop Sci., 11, 77 - 89.